Local tissue effects of various barrier membranes in a rat subcutaneous model
Naenni Nadja, 임현창, Strauss Franz-Josef, Jung Ronald E., Hammerle Christoph H. F., Thoma Daniel S.,
소속 상세정보
( Naenni Nadja ) - University of Zurich Clinic of Reconstructive Dentistry
임현창 ( Lim Hyun-Chang ) - Kyung Hee University School of Dentistry Department of Periodontology
( Strauss Franz-Josef ) - University of Zurich Clinic of Reconstructive Dentistry
( Jung Ronald E. ) - University of Zurich Clinic of Reconstructive Dentistry
( Hammerle Christoph H. F. ) - University of Zurich Clinic of Reconstructive Dentistry
( Thoma Daniel S. ) - University of Zurich Clinic of Reconstructive Dentistry
Abstract
Purpose: The purpose of this study was to examine the local tissue reactions associated with 3 different poly(lactic-co-glycolic acid) (PLGA) prototype membranes and to compare them to the reactions associated with commercially available resorbable membranes in rats.
Methods: Seven different membranes?3 synthetic PLGA prototypes (T1, T2, and T3) and 4 commercially available membranes (a PLGA membrane, a poly[lactic acid] membrane, a native collagen membrane, and a cross-linked collagen membrane)?were randomly inserted into 6 unconnected subcutaneous pouches in the backs of 42 rats. The animals were sacrificed at 4, 13, and 26 weeks. Descriptive histologic and histomorphometric assessments were performed to evaluate membrane degradation, visibility, tissue integration, tissue ingrowth, neovascularization, encapsulation, and inflammation. Means and standard deviations were calculated.
Results: The histological analysis revealed complete integration and tissue ingrowth of PLGA prototype T1 at 26 weeks. In contrast, the T2 and T3 prototypes displayed slight to moderate integration and tissue ingrowth regardless of time point. The degradation patterns of the 3 synthetic prototypes were similar at 4 and 13 weeks, but differed at 26 weeks. T1 showed marked degradation at 26 weeks, whereas T2 and T3 displayed moderate degradation. Inflammatory cells were present in all 3 prototype membranes at all time points, and these membranes did not meaningfully differ from commercially available membranes with regard to the extent of inflammatory cell infiltration.
Conclusions: The 3 PLGA prototypes, particularly T1, induced favorable tissue integration, exhibited a similar degradation rate to native collagen membranes, and elicited a similar inflammatory response to commercially available non?cross-linked resorbable membranes. The intensity of inflammation associated with degradable dental membranes appears to relate to their degradation kinetics, irrespective of their material composition.
키워드
Guided tissue regeneration; Bone regeneration; Materials testing; Polylactic acid-polyglycolic acid copolymer; Dental implants
원문 및 링크아웃 정보
등재저널 정보